AmethodformicrobialdecontaminationofAcanthamoebaculturesusingtheperitonealcavityofmice

摘要： Objective:ToevaluatewhethertheinoculationofcontaminatedculturesintheperitonealcavityofmicecouldimplementdecontaminationofAcanthamoebacultures.Methods:SuspensionsofAcanthamoeba,AcanthamoebapolyphagaATCC30461,orAcanthamoebaspp.isolatedfromsoil(UnB13strain)wereinoculatedintheperitonealcavityofSwissmice(n=24).After1,6,12,or24hofexposuretheperitonealcavitywaswashedandassessedforthepresenceofbacteria,fungi,andAcanthamoeba.Results:After1hofintraperitonealinoculationatleast97%ofthebacteriaand96%ofthefungi(P<0.05)and99%ofthebacteria(P<0.05)weresuccessfullyeliminatedfromtheATCC30461strainandfromthesoilisolateUnB13strain,respectively.ThismethodalsoallowedtherecoveryofmosttrophozoitesandcystsfrombothAcanthamoebaculturesattheendof24h.Conclusions:OurdatademonstratedthatthistechniquehasgreatpotentialfordecontaminationofAcanthamoebaculturesinashortperiodoftime. （共5页）